User:Beatriz Gimenez De C./Notebook/572/2015/01/27: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
No edit summary
Line 7: Line 7:
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==Tasks==
==Tasks==
* Produce the lysozyme AuNP fibers.  
* Produce the lysozyme AuNP fibers.
* Weigh the protease
** Made 50, 5 mL samples
* Weigh 4 mg of Pepsine protease into 8 tubes
* Prepare buffer


==Procedure==
==Procedure==


* Lysozyme-AuNP fibers preparation  
* Lysozyme-AuNP fibers preparation  
** Prepare Lysozyme (0.6 g/L lysozyme) stock solution
** Prepare Lysozyme 20 μM stock solution
** Prepare 2.5×10<sup>-3</sup> M HAuCl<sub>4</sub> stock solution
** Prepare 2.5×10<sup>-3</sup> M HAuCl<sub>4</sub> stock solution
** Transfer 0.5 mL 2.5×10<sup>-3</sup> M HAuCl<sub>4</sub> into x culture tubes
** Transfer 0.5 mL 2.5×10<sup>-3</sup> M HAuCl<sub>4</sub> into 50 culture tubes
*** 0.5×10<sup>-3</sup>(L)*2.5×10<sup>-3</sup>(mol/L)= 1.25 (mol) needed for A 45:1 ratio
*** 0.5×10<sup>-3</sup>(L)*2.5×10<sup>-3</sup>(mol/L)= 1.25 (mol) needed for A 45:1 ratio
** Transfer 0.661769570 mL of lysozyme stock solution into x culture tubes
** Transfer 1.39 mL of lysozyme stock solution into 50 culture tubes
*** 0.6(g/l)/14307(g/mol)= 4.1937510×10<sup>-5</sup>(mol/L)
*** 20*10^-6(mol/ml)/ 2.77777778×10<sup>-8</sup>(mol)= 1.39 mL for a 45:1 ratio
*** 2.77777778×10<sup>-8</sup>(mol)/4.1937510×10<sup>-5</sup>(mol/L)= 0.661769570 mL for a 45:1 ratio
** Fill up 50 culture tubes to 5 mL total with distilled water
** Fill up culture tubes to 5 mL total with water
** Placed in oven at 80ºC for 4h
** Placed in oven at 80ºC for 4h


Revision as of 00:00, 28 January 2015

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Tasks

  • Produce the lysozyme AuNP fibers.
    • Made 50, 5 mL samples
  • Weigh 4 mg of Pepsine protease into 8 tubes
  • Prepare buffer

Procedure

  • Lysozyme-AuNP fibers preparation
    • Prepare Lysozyme 20 μM stock solution
    • Prepare 2.5×10-3 M HAuCl4 stock solution
    • Transfer 0.5 mL 2.5×10-3 M HAuCl4 into 50 culture tubes
      • 0.5×10-3(L)*2.5×10-3(mol/L)= 1.25 (mol) needed for A 45:1 ratio
    • Transfer 1.39 mL of lysozyme stock solution into 50 culture tubes
      • 20*10^-6(mol/ml)/ 2.77777778×10-8(mol)= 1.39 mL for a 45:1 ratio
    • Fill up 50 culture tubes to 5 mL total with distilled water
    • Placed in oven at 80ºC for 4h



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Beatriz_Gimenez_De_C./Notebook/572/2015/01/27&oldid=855370"