User:Beatriz Gimenez De C./Notebook/572/2015/01/27: Difference between revisions
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==Tasks== | ==Tasks== | ||
* Produce the lysozyme AuNP fibers | * Produce the lysozyme AuNP fibers (Made 50, 5 mL samples) | ||
* Weigh 4 mg of Pepsine protease into 8 tubes | * Weigh 4 mg of Pepsine protease into 8 tubes | ||
* Prepare buffer | * Prepare buffer | ||
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** Prepare 2.5×10<sup>-3</sup> M HAuCl<sub>4</sub> stock solution | ** Prepare 2.5×10<sup>-3</sup> M HAuCl<sub>4</sub> stock solution | ||
** Transfer 0.5 mL 2.5×10<sup>-3</sup> M HAuCl<sub>4</sub> into 50 culture tubes | ** Transfer 0.5 mL 2.5×10<sup>-3</sup> M HAuCl<sub>4</sub> into 50 culture tubes | ||
*** 0.5×10<sup>-3</sup>(L)*2.5×10<sup>-3</sup>(mol/L)= 1.25 (mol) needed for | *** 0.5×10<sup>-3</sup>(L)*2.5×10<sup>-3</sup>(mol/L)= 1.25 (mol) needed for a 45:1 ratio | ||
** Transfer 1.39 mL of lysozyme stock solution into 50 culture tubes | ** Transfer 1.39 mL of lysozyme stock solution into 50 culture tubes | ||
*** 2.77777778×10<sup>-8</sup>(mol)/20*10^-6(mol/ml)= 1.39 mL for a 45:1 ratio | *** 2.77777778×10<sup>-8</sup>(mol)/20*10^-6(mol/ml)= 1.39 mL for a 45:1 ratio |
Revision as of 00:13, 28 January 2015
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Tasks
Procedure
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