User:Beatriz Gimenez De C./Notebook/572/2015/01/27: Difference between revisions
From OpenWetWare
No edit summary |
No edit summary |
||
(5 intermediate revisions by the same user not shown) | |||
Line 7: | Line 7: | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
==Tasks== | ==Tasks== | ||
* Produce the lysozyme AuNP fibers | * Produce the lysozyme AuNP fibers (Made 50, 5 mL samples) | ||
* Weigh | * Weigh 4 mg of Pepsine protease into 8 tubes | ||
* Prepare buffer | |||
==Procedure== | ==Procedure== | ||
* Lysozyme-AuNP fibers preparation | * Lysozyme-AuNP fibers preparation | ||
** Prepare Lysozyme | ** Prepare Lysozyme 20 μM stock solution | ||
*** Measured 0.0261 g of lysozyme into a 100 mL volumetric flask and filled the rest with distilled water | |||
** Prepare 2.5×10<sup>-3</sup> M HAuCl<sub>4</sub> stock solution | ** Prepare 2.5×10<sup>-3</sup> M HAuCl<sub>4</sub> stock solution | ||
** Transfer 0.5 mL 2.5×10<sup>-3</sup> M HAuCl<sub>4</sub> into | *** added 0.08539 g of HAuCl4 into a 100 mL volumetric flask and filled the rest with distilled water | ||
*** 0.5×10<sup>-3</sup>(L)*2.5×10<sup>-3</sup>(mol/L)= 1.25 (mol) needed for | ** Transfer 0.5 mL 2.5×10<sup>-3</sup> M HAuCl<sub>4</sub> into 50 culture tubes | ||
** Transfer | *** 0.5×10<sup>-3</sup>(L)*2.5×10<sup>-3</sup>(mol/L)= 1.25 (mol) needed for a 45:1 ratio | ||
** Transfer 1.39 mL of lysozyme stock solution into 50 culture tubes | |||
*** 2.77777778×10<sup>-8</sup>(mol)/ | *** 2.77777778×10<sup>-8</sup>(mol)/20*10^-6(mol/ml)= 1.39 mL for a 45:1 ratio | ||
** Fill up culture tubes to 5 mL total with water | ** Fill up 50 culture tubes to 5 mL total with distilled water | ||
** Placed in oven at 80ºC for 4h | ** Placed in oven at 80ºC for 4h | ||
*Weighed Protease pepsin into 8 tubes, for the next experiment. | |||
** 1. 0.00530 g | |||
** 2. 0.00398 g | |||
** 3. 0.00391 g | |||
** 4. 0.00470 g | |||
** 5. 0.00419 g | |||
** 6. 0.00465 g | |||
** 7. 0.00780 g | |||
** 8. 0.00584 g | |||
* Prepared 5 mM Tris/0.2 M NaCl buffer for reaction of the synthesized lysozyme-AuNP fibers with the proteases | |||
** 1000 mL of buffer was prepared, used 11.68960 g of NaCl + 0.78922 g of Tris in approx. 1000 mL of distilled water. | |||
** 0.5 N HCl added until pH=8.067. | |||
Revision as of 00:19, 28 January 2015
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Tasks
Procedure
|