Tasks
- Produce the lysozyme AuNP fibers.
- Weigh 4 mg of Pepsine protease into 8 tubes
- Prepare buffer
Procedure
- Lysozyme-AuNP fibers preparation
- Prepare Lysozyme 20 μM stock solution
- Prepare 2.5×10-3 M HAuCl4 stock solution
- Transfer 0.5 mL 2.5×10-3 M HAuCl4 into 50 culture tubes
- 0.5×10-3(L)*2.5×10-3(mol/L)= 1.25 (mol) needed for A 45:1 ratio
- Transfer 1.39 mL of lysozyme stock solution into 50 culture tubes
- 20*10^-6(mol/ml)/ 2.77777778×10-8(mol)= 1.39 mL for a 45:1 ratio
- Fill up 50 culture tubes to 5 mL total with distilled water
- Placed in oven at 80ºC for 4h
|