User:Beatriz Gimenez De C./Notebook/572/2015/01/27

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Tasks

  • Produce the lysozyme AuNP fibers.
    • Made 50, 5 mL samples
  • Weigh 4 mg of Pepsine protease into 8 tubes
  • Prepare buffer

Procedure

  • Lysozyme-AuNP fibers preparation
    • Prepare Lysozyme 20 μM stock solution
    • Prepare 2.5×10-3 M HAuCl4 stock solution
    • Transfer 0.5 mL 2.5×10-3 M HAuCl4 into 50 culture tubes
      • 0.5×10-3(L)*2.5×10-3(mol/L)= 1.25 (mol) needed for A 45:1 ratio
    • Transfer 1.39 mL of lysozyme stock solution into 50 culture tubes
      • 2.77777778×10-8(mol)/20*10^-6(mol/ml)= 1.39 mL for a 45:1 ratio
    • Fill up 50 culture tubes to 5 mL total with distilled water
    • Placed in oven at 80ºC for 4h



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