User:Beatriz Gimenez De C./Notebook/572/2015/02/03

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Task Data collection of reaction started o Wednesday 28th determine color change Set new protease reaction and monitor periodically Observations of samples set on the 28th Color change was only present for 57.8 μM pepsin concentration. It took 2 days to be apparent. Preparation of protease reactions Pepsin Prepare 115.6 μM of pepsin (34600 g/mol) stock solution with 16 mg in 4 ml of H2O 1- 2.5 ml of stock solution + 2.5 mL of buffer ( for a 57.8 μM Pepsin Concentration) 2- 0.5 mL of stock solution + 4.5 buffer (for a 11.56 μM Pepsin Concentration) 3- 0.05 mL of stock solution + 4.95 buffer (for a 1.156 μM Pepsin Concentration) 4- 0.005 mL of stock solution + 5 mL buffer (for a 0.1156 μM Pepsin Concentration) Trypsin Prepare 115.6 μM of trypsin stock solution with 10.77 mg in 4 ml H2O 1- 2.5 ml of stock solution + 2.5 mL of buffer ( for a 57.8 μM Trypsin Concentration) 2- 0.5 mL of stock solution + 4.5 buffer (for a 11.56 μM Trypsin Concentration) 3- 0.05 mL of stock solution + 4.95 buffer (for a 1.156 μM Trypsin Concentration) 4- 0.005 mL of stock solution + 5 mL buffer (for a 0.1156 μM Trypsin Concentration) α-Chymotrypsin Prepare 115.6 μM of α-Chymotrypsin stock solution with 11.56 mg in 4 ml H2O 1- 2.5 ml of stock solution + 2.5 mL of buffer ( for a 57.8 μM α-Chymotrypsin Concentration) 2- 0.5 mL of stock solution + 4.5 buffer (for a 11.56 μM α-Chymotrypsin Concentration) 3- 0.05 mL of stock solution + 4.95 buffer (for a 1.156 μM α-Chymotrypsin Concentration) 4- 0.005 mL of stock solution + 5 mL buffer (for a 0.1156 μM α-Chymotrypsin Concentration) Proteinase K Prepare 115.6 μM of Proteinase K stock solution with 13.37 mg in 4 ml H2O 1- 2.5 ml of stock solution + 2.5 mL of buffer ( for a 57.8 μM Proteinase K Concentration) 2- 0.5 mL of stock solution + 4.5 buffer (for a 11.56 μM Proteinase K Concentration) 3- 0.05 mL of stock solution + 4.95 buffer (for a 1.156 μM Proteinase K Concentration) 4- 0.005 mL of stock solution + 5 mL buffer (for a 0.1156 μM Proteinase K Concentration) Observations of reactions set today A instantaneous color change was seen with proteinase K at 57.8 μM, at11.56 μM and at 1.156 μM concentrations.