User:Beatriz Gimenez De C./Notebook/572/2015/03/25: Difference between revisions

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==Entry title==
==Tasks==
* Insert content here...
 
* Run Bradford analysis of the thermolysin samples run [http://openwetware.org/wiki/User:Monika_Gasiorek/Notebook/CHEM-571_2014F/2015/03/24 Tuesday 24]
** 200μM of a 1 in 4 Bradford dilution (1 mL Bradford in 3 mL of Tris/NaCl buffer)
** 150μM of each thermolysin sample
** 550μM of tris/NaCl buffer
 
==Results==
 
* Bradford results corrected for proteinase K
 
[[Image:Proteinase_K_Bradford_2_1_uM.png]]
 
[[Image:Proteinase_K_Bradford_2_100_nM.png]]
 
[[Image:Proteinase_K_Bradford_2_10_nM.png]]
 
[[Image:Proteinase_K_Bradford_2_1_nM.png]]





Revision as of 22:23, 28 March 2015

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Tasks

  • Run Bradford analysis of the thermolysin samples run Tuesday 24
    • 200μM of a 1 in 4 Bradford dilution (1 mL Bradford in 3 mL of Tris/NaCl buffer)
    • 150μM of each thermolysin sample
    • 550μM of tris/NaCl buffer

Results

  • Bradford results corrected for proteinase K