User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli

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==04/04/11==
==04/04/11==
<!-- Precede finished items with a checkmark &#x2713; -->
<!-- Precede finished items with a checkmark &#x2713; -->
-
* &#x2713; Assemblies: BD111, BD112
+
* &#x2713; Assemblies: BD111, BD112, BD113
----
----
'''Assemblies'''
'''Assemblies'''
-
# KAH213: (4) KAH54/(S/P)/430 + (1) KAH210/(X/P)/2398
+
# BD111: KAH109/(X/P)/1123 + BBa_B0030/(S/P)/15
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# KAH214: " + (2) KAH211/(X/P)/1321
+
# BD112: BD111/(X/P)/1138 + BBa_I712074/(S/P)46
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# KAH215: " + (3) KAH212/(X/P)/1621
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# BD113: BD111/(X/P)/1138 + BBa_I719005/(S/P)23
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# KAH216: hPCD/(S/P)/186 &#x2713; + KAH210/(X/P)/2398
+
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# KAH217: " + KAH211/(X/P)/1321
+
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# KAH218: " + KAH212/(X/P)/1621
+
-
# KAH207/V0200: KAH207/(N/S)/1836 &#x2713; + V0200/(N/S dp)/4442
+
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# KAH208/V0200: KAH208/(N/S)/1884 &#x2713; + "
+
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# KAH209/V0200: KAH209/(N/S)/1932 &#x2713; + "
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| rowspan="9" | [[Image:KAH_071211_gel1.tif|200px|Cloning digests 7/12/11]]<br>30 μL/lane, 1% agarose -->
| rowspan="9" | [[Image:KAH_071211_gel1.tif|200px|Cloning digests 7/12/11]]<br>30 μL/lane, 1% agarose -->
|-
|-
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| DNA (plasmid) || up to 25 μL
+
| DNA (plasmid) || up to 20 μL
|-
|-
| 10x buffer || 3.0
| 10x buffer || 3.0

Revision as of 20:47, 3 April 2012

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04/04/11

  • ✓ Assemblies: BD111, BD112, BD113

Assemblies

  1. BD111: KAH109/(X/P)/1123 + BBa_B0030/(S/P)/15
  2. BD112: BD111/(X/P)/1138 + BBa_I712074/(S/P)46
  3. BD113: BD111/(X/P)/1138 + BBa_I719005/(S/P)23


> Digests (Fermentas FD)

Reagent Volume   Cloning digests 7/12/11
30 μL/lane, 1% agarose -->
DNA (plasmid) up to 20 μL
10x buffer 3.0
enzyme 1 1.0
enzyme 2 1.0
dH2O ---
  30 μL --> 37°C/ ~30 min.


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