User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli

< User:Behzad Damadzadeh | Notebook(Difference between revisions)

Current revision

Project Description

@@ Line 13: / Line 13: @@
 |-
 |colspan="2"|
-==04/04/11==
-<!-- Precede finished items with a checkmark &#x2713; -->
-* &#x2713; Assemblies: BD111, BD112
-----
-'''Assemblies'''
-# KAH213: (4) KAH54/(S/P)/430 + (1) KAH210/(X/P)/2398
-# KAH214: " + (2) KAH211/(X/P)/1321
-# KAH215: " + (3) KAH212/(X/P)/1621
-# KAH216: hPCD/(S/P)/186 &#x2713; + KAH210/(X/P)/2398
-# KAH217: " + KAH211/(X/P)/1321
-# KAH218: " + KAH212/(X/P)/1621
-# KAH207/V0200: KAH207/(N/S)/1836 &#x2713; + V0200/(N/S dp)/4442
-# KAH208/V0200: KAH208/(N/S)/1884 &#x2713; + "
-# KAH209/V0200: KAH209/(N/S)/1932 &#x2713; + "
+==Behzad's Notebook==
-> Digests (Fermentas FD)<br>
+'''Project Description'''
+* Project description coming soon
-{| class="wikitable" border="0" cellspacing="3" <!-- Digest rxn. table -->
-|-valign="top"
-| <u>Reagent</u> || <u>Volume</u> || &nbsp;
-| rowspan="9" | [[Image:KAH_071211_gel1.tif|200px|Cloning digests 7/12/11]]<br>30 μL/lane, 1% agarose -->
-|-
-| DNA (plasmid) || up to 25 μL
-|-
-| 10x buffer || 3.0
-|-
-| enzyme 1 || 1.0
-|-
-| enzyme 2 || 1.0
-|-
-| dH<sub>2</sub>O || ---
-|-
-| &nbsp; || 30 μL --> 37°C/ ~30 min.
-|}
-__NOTOC__