User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli

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04/04/11

  • ✓ Assemblies: BD111, BD112

Assemblies

  1. KAH213: (4) KAH54/(S/P)/430 + (1) KAH210/(X/P)/2398
  2. KAH214: " + (2) KAH211/(X/P)/1321
  3. KAH215: " + (3) KAH212/(X/P)/1621
  4. KAH216: hPCD/(S/P)/186 ✓ + KAH210/(X/P)/2398
  5. KAH217: " + KAH211/(X/P)/1321
  6. KAH218: " + KAH212/(X/P)/1621
  7. KAH207/V0200: KAH207/(N/S)/1836 ✓ + V0200/(N/S dp)/4442
  8. KAH208/V0200: KAH208/(N/S)/1884 ✓ + "
  9. KAH209/V0200: KAH209/(N/S)/1932 ✓ + "


> Digests (Fermentas FD)

Reagent Volume   Cloning digests 7/12/11
30 μL/lane, 1% agarose -->
DNA (plasmid) up to 25 μL
10x buffer 3.0
enzyme 1 1.0
enzyme 2 1.0
dH2O ---
  30 μL --> 37°C/ ~30 min.


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