User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2012/04/04: Difference between revisions
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'''Separate the fragments via gel electrophoresis and purify the fragments''' | '''Separate the fragments via gel electrophoresis and purify the fragments''' | ||
[[Image:PcTF-RBS-Digestion-Gel-Behzad4-4-12.jpg|thumb|350px|White dashed lines border where the gel was cut to excise vector fragment (RBS) and insert fragment (PcTF).]] | |||
# Make a 0.8% gel: add 0.5 g agarose to ~60 ml 1x TAE buffer in a glass flask. | # Make a 0.8% gel: add 0.5 g agarose to ~60 ml 1x TAE buffer in a glass flask. | ||
# Mix by swirling and microwave for 40 seconds. Mix by swirling again (to eliminate air pockets and prevent boiling-over) and microwave for 40 seconds. | # Mix by swirling and microwave for 40 seconds. Mix by swirling again (to eliminate air pockets and prevent boiling-over) and microwave for 40 seconds. | ||
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__NOTOC__ | __NOTOC__ | ||
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'''DNA Re-Transformation''' | |||
[[category:OWWLabNotebookV1]] | [[category:OWWLabNotebookV1]] |
Revision as of 19:03, 4 April 2012
PcTF Subcloning in E. coli | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||
04/04/12General plan for assemblies
Assemblies
> Digests (Fermentas FD)
Separate the fragments via gel electrophoresis and purify the fragments
DNA Re-Transformation |