User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2012/04/05

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
Line 10: Line 10:
'''Culturing the colonies grew on agar plates on 4/4/2012'''
'''Culturing the colonies grew on agar plates on 4/4/2012'''
-
# BBa_I712074
+
# BBa_I712074 (Re-transformed gene)
-
# BBa_I719005
+
# BBa_I719005 (Re-transformed gene)
-
# BBa_B0030
+
# BBa_B0030 (Re-transformed gene)
-
# BD111
+
# BD111 (Constructed gene)
'''Grow liquid cultures'''
'''Grow liquid cultures'''

Revision as of 14:41, 5 April 2012

PcTF Subcloning in E. coli Main project page
Previous entry      Next entry

5/4/2012

Culturing the colonies grew on agar plates on 4/4/2012

  1. BBa_I712074 (Re-transformed gene)
  2. BBa_I719005 (Re-transformed gene)
  3. BBa_B0030 (Re-transformed gene)
  4. BD111 (Constructed gene)

Grow liquid cultures

  1. Label 15 ml sterile culture tube(s) appropriately. Fill each tube with 2 ml of LB growth medium + appropriate antibiotic (e.g., 100 μg/ml ampicillin).
  2. Using a sterile pipette tip, touch the bacterial streak (or pick up a single colony) and put the tip into the LB medium (bacterial end down).
  3. Grow the cultures for 7 hours in a shaking 37°C incubator.

Personal tools