User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2012/04/05: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
No edit summary
Line 10: Line 10:


'''Culturing the colonies grew on agar plates on 4/4/2012'''
'''Culturing the colonies grew on agar plates on 4/4/2012'''
# BBa_I712074
# BBa_I712074 (Re-transformed gene)
# BBa_I719005
# BBa_I719005 (Re-transformed gene)
# BBa_B0030
# BBa_B0030 (Re-transformed gene)
# BD111
# BD111 (Constructed gene)


'''Grow liquid cultures'''
'''Grow liquid cultures'''

Revision as of 11:41, 5 April 2012

PcTF Subcloning in E. coli <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

5/4/2012

Culturing the colonies grew on agar plates on 4/4/2012

  1. BBa_I712074 (Re-transformed gene)
  2. BBa_I719005 (Re-transformed gene)
  3. BBa_B0030 (Re-transformed gene)
  4. BD111 (Constructed gene)

Grow liquid cultures

  1. Label 15 ml sterile culture tube(s) appropriately. Fill each tube with 2 ml of LB growth medium + appropriate antibiotic (e.g., 100 μg/ml ampicillin).
  2. Using a sterile pipette tip, touch the bacterial streak (or pick up a single colony) and put the tip into the LB medium (bacterial end down).
  3. Grow the cultures for 7 hours in a shaking 37°C incubator.
Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Behzad_Damadzadeh/Notebook/PcTF_Subcloning_in_E-coli/2012/04/05&oldid=596222"