User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2012/04/05

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5/4/2012

Culturing the colonies grew on agar plates on 4/4/2012

  1. BBa_I712074
  2. BBa_I719005
  3. BBa_B0030
  4. BD111

Grow liquid cultures

  1. Label 15 ml sterile culture tube(s) appropriately. Fill each tube with 2 ml of LB growth medium + appropriate antibiotic (e.g., 100 μg/ml ampicillin).
  2. Using a sterile pipette tip, touch the bacterial streak (or pick up a single colony) and put the tip into the LB medium (bacterial end down).
  3. Grow the cultures for 7 hours in a shaking 37°C incubator.

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