User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2012/04/08: Difference between revisions
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# Incubate overnight at 37°C to get colonies | # Incubate overnight at 37°C to get colonies | ||
Note: The negative control will show you the number of “background” colonies so that you can determine whether your transformation worked, or is just the result of vector self-ligation or selection failure. | Note: The negative control will show you the number of “background” colonies so that you can determine whether your transformation worked, or is just the result of vector self-ligation or selection failure. | ||
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Revision as of 16:50, 8 April 2012
PcTF Subcloning in E. coli | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||
8/4/12Continued from 6/4/12
BD-112 assembly: Xng inser= (1144 / 3472) x 2 x 20 ng vector=14 Vectore= (20 ng/16μL)= 1.25 μL Insert= (14ng/8μL)=2 μL BD-113 assembly: Xng inser= (1144 / 2102) x 2 x 20 ng vector=21 Vectore= (20 ng/17μL)= 1.17 μL Insert= (21ng/8μL)=2 μL
Transform bacteria with the ligated plasmids 30 minutes
Note: The negative control will show you the number of “background” colonies so that you can determine whether your transformation worked, or is just the result of vector self-ligation or selection failure. |