User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2012/04/11

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(Autocreate 2012/04/11 Entry for User:Behzad_Damadzadeh/Notebook/PcTF_Subcloning_in_E-coli)
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=Date=
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=11/4/12=
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'''List title'''
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> Digests (Fermentas FD)<br>
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# List items
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BD-112 and BD-113
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[[Image:BD-111-Behzad-5-4-12.jpg|thumb|350px|White dashed lines border show the vector backbone and the BD-111 constructed gene(RBS + PcTF).]]
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{| class="wikitable" width=400px
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| Plasmid DNA || 2.0 μl*
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|-
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| EcoR1  || 1.0 μl
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|-
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| Pst1 || 1.0 μl
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|-
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| 10x FastDigest buffer + green loading dye || 1.5 μl
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|-
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| dH<sub>2</sub>O || 9.5 μl
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|-
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| &nbsp; || 15.0 μl total
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|-
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| Incubate at 37°C for 10 minutes.
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|}
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# Make the (1%) agarose glee and add 15μl  of restricted DNA (PcTF & RBS) in one well and 10 μl  of ladder.
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'The size of the constructed gene supposed to be: 1123 + 15 + 6 = 1144 bp'

Revision as of 12:00, 11 April 2012

PcTF Subcloning in E. coli Main project page
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11/4/12

> Digests (Fermentas FD)
BD-112 and BD-113

White dashed lines border show the vector backbone and the BD-111 constructed gene(RBS + PcTF).
White dashed lines border show the vector backbone and the BD-111 constructed gene(RBS + PcTF).
Plasmid DNA 2.0 μl*
EcoR1 1.0 μl
Pst1 1.0 μl
10x FastDigest buffer + green loading dye 1.5 μl
dH2O 9.5 μl
  15.0 μl total
Incubate at 37°C for 10 minutes.
  1. Make the (1%) agarose glee and add 15μl of restricted DNA (PcTF & RBS) in one well and 10 μl of ladder.

'The size of the constructed gene supposed to be: 1123 + 15 + 6 = 1144 bp'

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