User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2012/04/11 - Revision history

Behzad Damadzadeh at 20:57, 11 April 2012

2012-04-11T20:57:10Z

←Older revision		Revision as of 20:57, 11 April 2012
Line 12:		Line 12:
	BD-112 and BD-113		BD-112 and BD-113

-	[[Image:~~BD-111~~-~~Behzad~~-~~5-4-12~~.~~jpg~~\|thumb\|350px\|White dashed lines border show the vector backbone and the BD-~~111~~ constructed gene(RBS + PcTF).]]	+	[[Image:BD112-BD113-Gel.JPG\|thumb\|350px\|2 colonies of BD-112 (1 & 2) and 2 colonies of BD-113 (3 & 4)- White dashed lines border, 1 and 2 show the vector backbone (3426 bp) and the BD-112 constructed gene(T7 + RBS + PcTF + 2 scars) (1196 bp), 3 and 4 show the vector backbone (2079 bp) and the BD-113 constructed gene(T7 + RBS + PcTF + 2 scars) (1196 bp).]]
	{\| class="wikitable" width=400px		{\| class="wikitable" width=400px
	\| Plasmid DNA \|\| 2.0 μl*		\| Plasmid DNA \|\| 2.0 μl*
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	# Make the (1%) agarose glee and add 15μl of restricted DNA (PcTF & RBS) in one well and 10 μl of ladder.		# Make the (1%) agarose glee and add 15μl of restricted DNA (PcTF & RBS) in one well and 10 μl of ladder.
-	'The size of the constructed gene supposed to be: 1123 + 15 + 6 = 1144 bp'	+	* The size of the constructed gene supposed to be: 1123 + 15 + 6 + 6 + 46 = 1144 bp'

Behzad Damadzadeh at 17:00, 11 April 2012

2012-04-11T17:00:00Z

←Older revision		Revision as of 17:00, 11 April 2012
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	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->

-	=~~Date~~=	+	=11/4/12=

-	~~'''List title'''~~	+	> Digests (Fermentas FD)<br>
-	# ~~List items~~	+	BD-112 and BD-113
		+
		+	[[Image:BD-111-Behzad-5-4-12.jpg\|thumb\|350px\|White dashed lines border show the vector backbone and the BD-111 constructed gene(RBS + PcTF).]]
		+	{\| class="wikitable" width=400px
		+	\| Plasmid DNA \|\| 2.0 μl*
		+	\|-
		+	\| EcoR1 \|\| 1.0 μl
		+	\|-
		+	\| Pst1 \|\| 1.0 μl
		+	\|-
		+	\| 10x FastDigest buffer + green loading dye \|\| 1.5 μl
		+	\|-
		+	\| dH<sub>2</sub>O \|\| 9.5 μl
		+	\|-
		+	\|   \|\| 15.0 μl total
		+	\|-
		+	\| Incubate at 37°C for 10 minutes.
		+	\|}
		+
		+	# Make the (1%) agarose glee and add 15μl of restricted DNA (PcTF & RBS) in one well and 10 μl of ladder.
		+	'The size of the constructed gene supposed to be: 1123 + 15 + 6 = 1144 bp'

Behzad Damadzadeh: Autocreate 2012/04/11 Entry for User:Behzad_Damadzadeh/Notebook/PcTF_Subcloning_in_E-coli

2012-04-11T16:53:46Z

Autocreate 2012/04/11 Entry for User:Behzad_Damadzadeh/Notebook/PcTF_Subcloning_in_E-coli

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{|{{table}} width="800"
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">PcTF Subcloning in E. coli</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|-
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=Date=

'''List title'''
# List items