User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2012/11/16: Difference between revisions
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| <u>Sample</u> || <u>OD260</u> || <u>260/280</u> || <u>ng/μL</u> | | <u>Sample</u> || <u>OD260</u> || <u>260/280</u> || <u>ng/μL</u> | ||
|- | |- | ||
| 1. pT7CFE1-CHis (E/P) || --- || --- || --- | | 1. pT7CFE1-CHis (E/P) || --- || 1.829 || 57.344 | ||
|- | |||
| 2. KAH109 (E/P) || --- || 2.354 || 36.072 | |||
|- | |||
| 3. KAH111 (E/P) || --- || 2.238 || 15.003 | |||
|- | |||
| 4. KAH112 (E/P) || --- || 1.862 || 22.857 | |||
|- | |||
| 5. KAH115 (E/P) || --- || 2.096 || 18.312 | |||
|- | |||
| 6. KAH148 (E/P) || --- || 2.389 || 23.735 | |||
|- | |||
| 7. KAH150 (E/P) || --- || 1.99 || 19.822 | |||
|- | |||
| 8. KAH151 (E/P) || --- || 1.9 || 19.701 | |||
|} | |} | ||
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| || 1 || 2 || 3 || 4 || 5 || 6 || 7 || 8 | | || 1 || 2 || 3 || 4 || 5 || 6 || 7 || 8 | ||
|- | |- | ||
| Insert | | Insert Name || KAH109 || KAH111 || KAH112 || KAH115 || KAH148 || KAH150 || KAH151 || N/A | ||
|- | |||
| Insert DNA || 1.3 || 3.12 || 2.05 || 2.12 || 1.7 || 2.03 || 2.05 || 0 | |||
|- | |- | ||
| Vector DNA || | | Vector DNA || 0.87 || 0.87 || 0.87 || 0.87 || 0.87 || 0.87 || 0.87 || 0.87 | ||
|- | |- | ||
| 2x lgn buf (Roche) || 5.0 || 5.0 || 5.0 || 5.0 || 5.0 || 5.0 || 5.0 || 5.0 | | 2x lgn buf (Roche) || 5.0 || 5.0 || 5.0 || 5.0 || 5.0 || 5.0 || 5.0 || 5.0 |
Revision as of 13:29, 26 November 2012
PcTF Subcloning in E. coli | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11/16/12
Assemblies: Shown as Part/cuts/purified fragment size
Measure concentration(s)
Ligations (trouble shooting suggestions)
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