User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2012/11/16: Difference between revisions
From OpenWetWare
Line 26: | Line 26: | ||
'''Digest (Fermentas FD)'''<br> | |||
# pT7CFE1-CHis, E/P | # pT7CFE1-CHis, E/P | ||
Line 49: | Line 49: | ||
'''Measure concentration(s)''' | |||
{| class="wikitable" border="0" cellspacing="3" <!-- [DNA] table --> | {| class="wikitable" border="0" cellspacing="3" <!-- [DNA] table --> | ||
|- | |- | ||
Line 59: | Line 59: | ||
'''Ligations''' (trouble shooting suggestions) | |||
* Note: Use 50 ng of vector this time (instead of 20) | * Note: Use 50 ng of vector this time (instead of 20) | ||
* Note: Use a 3:1 ratio of insert to vector... x μL insert = 3 * (bp of insert/ bp of vector) * 50 ng vector / (concentration of insert ng/μL) | * Note: Use a 3:1 ratio of insert to vector... x μL insert = 3 * (bp of insert/ bp of vector) * 50 ng vector / (concentration of insert ng/μL) |
Revision as of 12:07, 16 November 2012
PcTF Subcloning in E. coli | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11/16/12
Assemblies: Shown as Part/cuts/purified fragment size
Measure concentration(s)
Ligations (trouble shooting suggestions)
|