User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2012/11/16: Difference between revisions
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| 1. pT7CFE1-CHis (E/P) || --- || --- || --- | | 1. pT7CFE1-CHis (E/P) || --- || --- || --- | ||
|} | |||
> Ligations (trouble shooting suggestions) | |||
* Note: Use 50 ng of vector this time (instead of 20) | |||
* Note: Use a 3:1 ratio of insert to vector... x μL insert = 3 * (bp of insert/ bp of vector) * 50 ng vector / (concentration of insert ng/μL) | |||
{| class="wikitable" border="0" cellspacing="3" <!-- Ligation rxn table --> | |||
| || 1 || 2 || | |||
|- | |||
| Insert DNA || 3.5 || --- || | |||
|- | |||
| Vector DNA || 0.5 || 0.5 || | |||
|- | |||
| 2x lgn buf (Roche) || 5.0 || 5.0 || | |||
|- | |||
| T4 ligase (NEB) || 1.0 || 1.0 || | |||
|- | |||
| dH<sub>2</sub>O || --- || 3.5 || | |||
|- | |||
| || 10 μL || 10 μL || | |||
|} | |} |
Revision as of 12:01, 16 November 2012
PcTF Subcloning in E. coli | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||||||||
11/16/12
Assemblies: Shown as Part/cuts/purified fragment size
> Measure concentration(s)
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