User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2012/11/16: Difference between revisions
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| 1. pT7CFE1-CHis (E/P) || --- || --- || --- | | 1. pT7CFE1-CHis (E/P) || --- || --- || --- | ||
|} | |} | ||
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{| class="wikitable" border="0" cellspacing="3" <!-- Ligation rxn table --> | {| class="wikitable" border="0" cellspacing="3" <!-- Ligation rxn table --> | ||
| || 1 || 2 || | | || 1 || 2 || 3 || 4 || 5 || 6 || 7 || 8 | ||
|- | |- | ||
| Insert DNA || | | Insert DNA || --- || --- || --- || --- || --- || --- || --- || --- | ||
|- | |- | ||
| Vector DNA || | | Vector DNA || --- || --- || --- || --- || --- || --- || --- || --- | ||
|- | |- | ||
| 2x lgn buf (Roche) || 5.0 || 5.0 || | | 2x lgn buf (Roche) || 5.0 || 5.0 || 5.0 || 5.0 || 5.0 || 5.0 || 5.0 || 5.0 | ||
|- | |- | ||
| T4 ligase (NEB) || 1.0 || 1.0 || | | T4 ligase (NEB) || 1.0 || 1.0 || 1.0 || 1.0 || 1.0 || 1.0 || 1.0 || 1.0 | ||
|- | |- | ||
| dH<sub>2</sub>O || --- || | | dH<sub>2</sub>O || --- || --- || --- || --- || --- || --- || --- || --- | ||
|- | |- | ||
| || 10 μL || 10 μL || | | || 10 μL || 10 μL || 10 μL || 10 μL || 10 μL || 10 μL || 10 μL || 10 μL | ||
|} | |} |
Revision as of 12:03, 16 November 2012
PcTF Subcloning in E. coli | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11/16/12
Assemblies: Shown as Part/cuts/purified fragment size
> Measure concentration(s)
> Ligations (trouble shooting suggestions)
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