User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2012/12/01: Difference between revisions

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(Autocreate 2012/12/01 Entry for User:Behzad_Damadzadeh/Notebook/PcTF_Subcloning_in_E-coli)
 
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=Date=
=12/1/12=


'''List title'''
> Digests (Fermentas FD)<br>
# List items
PT7CFE1-CHis
 
[[Image:Vector_Cut_(E-P).png‎ |thumb|350px|2 Human Vector PT7CFE1-CHis, 3600 bp, Cut with EcoRI and PstI Separately.]]
{| class="wikitable" width=400px
| Plasmid DNA || 3.0 μl*
|-
| EcoR1  || 1.0 μl
|-
| Pst1 || 1.0 μl
|-
| 10x FastDigest buffer + green loading dye || 1.5 μl
|-
| dH<sub>2</sub>O || 8.5 μl
|-
| &nbsp; || 15.0 μl total
|-
| Incubate at 37°C for 10 minutes.
|}
 
# Make the (1%) agarose glee and add 15μl  of restricted vector in one well and 10 μl  of ladder.

Revision as of 19:41, 2 December 2012

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12/1/12

> Digests (Fermentas FD)
PT7CFE1-CHis

2 Human Vector PT7CFE1-CHis, 3600 bp, Cut with EcoRI and PstI Separately.
Plasmid DNA 3.0 μl*
EcoR1 1.0 μl
Pst1 1.0 μl
10x FastDigest buffer + green loading dye 1.5 μl
dH2O 8.5 μl
  15.0 μl total
Incubate at 37°C for 10 minutes.
  1. Make the (1%) agarose glee and add 15μl of restricted vector in one well and 10 μl of ladder.
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