User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2012/12/01

From OpenWetWare

< User:Behzad Damadzadeh | Notebook | PcTF Subcloning in E-coli | 2012 | 12
Revision as of 23:02, 2 December 2012 by Behzad Damadzadeh (Talk | contribs)
(diff) ←Older revision | Current revision (diff) | Newer revision→ (diff)
Jump to: navigation, search
PcTF Subcloning in E. coli Main project page
Previous entry      Next entry

12/1/12

> Digests (Fermentas FD)

PT7CFE1-CHis

Human Vector PT7CFE1-CHis, 3600 bp, Cut with EcoRI and PstI Separately.
Human Vector PT7CFE1-CHis, 3600 bp, Cut with EcoRI and PstI Separately.
Plasmid DNA 3.0 μl*
EcoR1 1.0 μl
Pst1 1.0 μl
10x FastDigest buffer + green loading dye 1.5 μl
dH2O 8.5 μl
  15.0 μl total
Incubate at 37°C for 10 minutes.
  1. Make the (1%) agarose glee and add 15μl of restricted vector in one well and 10 μl of ladder.
Personal tools