User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2012/12/14

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(Autocreate 2012/12/14 Entry for User:Behzad_Damadzadeh/Notebook/PcTF_Subcloning_in_E-coli)
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(14/12/2012)
 
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=Date=
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=14/12/2012=
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Ligation of KAH111 and C-His vector in NEB10B and BL21
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* Ligations
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{| {{table}} cellspacing="3" <!-- Ligations table -->
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|- bgcolor=#cfcfcf
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| Ligation || <font color="blue"><u>Plate results (lig : neg crtl)</u> 12/12/12</font>
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|-
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| 1. E. coli NEB10B KAH111(E/P)/size, 16 ng + C-His(E/P)/3600, 25ng || <font color="blue">KAH111/C-His 3:1 No Colonies</font>
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|-
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| 2. E. coli BL-21  KAH111(E/P)/size, 16 ng + C-His(E/P)/3600, 25ng || <font color="blue">KAH111/C-His 3:1 No Colonies</font>
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|-
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| 3. C-His(E/P)/ 25 ng ||
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|}
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'''List title'''
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{| {{table}} cellspacing="3" <!-- Ligation rxn table -->
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# List items
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| &nbsp;            || 1    || 2  || 3 
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|-
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| Insert DNA        || 3.12  || 3.12 || --- 
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|-
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| Vector DNA        || 2.94  || 2.94 || 2.94 
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|-
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| 2x lgn buf (Roche) || 7.06  || 7.06 || 5.0
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|-
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| T4 ligase (NEB)    || 1.0  || 1.0  || 1.0
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|-
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| dH<sub>2</sub>O    || 0 || 0 || 0.88 
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|-
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| &nbsp;            || 14.12 μL || 14.12 μL || 10μl
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|}

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14/12/2012

Ligation of KAH111 and C-His vector in NEB10B and BL21

  • Ligations
Ligation Plate results (lig : neg crtl) 12/12/12
1. E. coli NEB10B KAH111(E/P)/size, 16 ng + C-His(E/P)/3600, 25ng KAH111/C-His 3:1 No Colonies
2. E. coli BL-21 KAH111(E/P)/size, 16 ng + C-His(E/P)/3600, 25ng KAH111/C-His 3:1 No Colonies
3. C-His(E/P)/ 25 ng
  1 2 3
Insert DNA 3.12 3.12 ---
Vector DNA 2.94 2.94 2.94
2x lgn buf (Roche) 7.06 7.06 5.0
T4 ligase (NEB) 1.0 1.0 1.0
dH2O 0 0 0.88
  14.12 μL 14.12 μL 10μl
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