User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2012/12/14: Difference between revisions
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= | =14/12/2012= | ||
* Ligations | |||
# | {| {{table}} cellspacing="3" <!-- Ligations table --> | ||
|- bgcolor=#cfcfcf | |||
| Ligation || <font color="blue"><u>Plate results (lig : neg crtl)</u> 12/12/12</font> | |||
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| 1. E. coli NEB10B KAH111(E/P)/size, 16 ng + C-His(E/P)/3600, 25ng || <font color="blue">KAH111/C-His 3:1 (Pick 4)</font> | |||
|- | |||
| 2. E. coli BL-21 KAH111(E/P)/size, 16 ng + C-His(E/P)/3600, 25ng || <font color="blue">KAH111/C-His 3:1 (Pick 4)</font> | |||
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| 3. C-His(E/P)/ 25 ng || | |||
|} | |||
{| {{table}} cellspacing="3" <!-- Ligation rxn table --> | |||
| || 1 || 2 || 3 | |||
|- | |||
| Insert DNA || 3.12 || 3.12 || --- | |||
|- | |||
| Vector DNA || 2.94 || 2.94 || 2.94 | |||
|- | |||
| 2x lgn buf (Roche) || 7.06 || 7.06 || 5.0 | |||
|- | |||
| T4 ligase (NEB) || 1.0 || 1.0 || 1.0 | |||
|- | |||
| dH<sub>2</sub>O || 0 || 0 || 0.88 | |||
|- | |||
| || 14.12 μL || 14.12 μL || 10μl | |||
|} |
Revision as of 16:52, 14 December 2012
PcTF Subcloning in E. coli | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||
14/12/2012
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