User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2012/12/14
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| Ligation || <font color="blue"><u>Plate results (lig : neg crtl)</u> 12/12/12</font> | | Ligation || <font color="blue"><u>Plate results (lig : neg crtl)</u> 12/12/12</font> | ||
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| - | | 1. E. coli NEB10B KAH111(E/P)/size, 16 ng + C-His(E/P)/3600, 25ng || <font color="blue">KAH111/C-His 3:1 | + | | 1. E. coli NEB10B KAH111(E/P)/size, 16 ng + C-His(E/P)/3600, 25ng || <font color="blue">KAH111/C-His 3:1 No Colonies</font> |
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| - | | 2. E. coli BL-21 KAH111(E/P)/size, 16 ng + C-His(E/P)/3600, 25ng || <font color="blue">KAH111/C-His 3:1 | + | | 2. E. coli BL-21 KAH111(E/P)/size, 16 ng + C-His(E/P)/3600, 25ng || <font color="blue">KAH111/C-His 3:1 No Colonies</font> |
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| 3. C-His(E/P)/ 25 ng || | | 3. C-His(E/P)/ 25 ng || | ||
Current revision
 PcTF Subcloning in E. coli
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14/12/2012Ligation of KAH111 and C-His vector in NEB10B and BL21
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