User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2012/12/18: Difference between revisions
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''' | '''RFP Plate Reader Serial Dilution''' | ||
# | # Transfer 100 μl of RFP from stock to 100μl DI water. (50% solution) | ||
# Transfer 100 μl of 50% solution to 100 μl DI water. (25% solution) | |||
# Transfer 100 μl of 25% solution to 100 μl DI water. (12.5% solution) | |||
# Transfer 100 μl of 12.5% solution to 100 μl DI water. (6.25% solution) | |||
'''Plate Reader''' | |||
* Transfer 50 μl of each solution to the Costar 96 clear bottom black side. | |||
* Here is the software setting: | |||
** Fluorescence | |||
** Endpoint | |||
** Full Plate | |||
** Excitation: 540, Emission: 600 | |||
** Optics: Top | |||
** Gain: AutoScale | |||
** Light Source: Xenon Flash, Lamp Energy: High | |||
** Read Speed: Normal, Delay: 100 msec, Measurements/Data Point: 10 | |||
** Read Height: 9.5 mm |
Revision as of 14:02, 18 December 2012
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18/12/2012RFP Plate Reader Serial Dilution
Plate Reader
|