User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2012/12/18

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Line 27: Line 27:
** Read Speed: Normal,  Delay: 100 msec,  Measurements/Data Point: 10
** Read Speed: Normal,  Delay: 100 msec,  Measurements/Data Point: 10
** Read Height: 9.5 mm
** Read Height: 9.5 mm
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* Click OK and then Run the experiment.
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'''Result'''
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{| border="1"
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! Header text !! Header text
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|-
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|format modifier (not displayed) | 100% ||43125
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|-
 +
|format  |50% ||format | 16143
 +
|-
 +
|format  |25% ||format | 7978
 +
|-
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|format  |12.5% ||format | 4124
 +
|-
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|format  |6.25% ||format | 2718
 +
|}

Revision as of 16:10, 18 December 2012

PcTF Subcloning in E. coli Main project page
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18/12/2012

RFP Plate Reader Serial Dilution

  1. Transfer 100 μl of RFP from stock to 100μl DI water. (50% solution)
  2. Transfer 100 μl of 50% solution to 100 μl DI water. (25% solution)
  3. Transfer 100 μl of 25% solution to 100 μl DI water. (12.5% solution)
  4. Transfer 100 μl of 12.5% solution to 100 μl DI water. (6.25% solution)

Plate Reader

  • Transfer 50 μl of each solution to the Costar 96 clear bottom black side.
  • Here is the software setting:
    • Fluorescence
    • Endpoint
    • Full Plate
    • Excitation: 540, Emission: 600
    • Optics: Top
    • Gain: AutoScale
    • Light Source: Xenon Flash, Lamp Energy: High
    • Read Speed: Normal, Delay: 100 msec, Measurements/Data Point: 10
    • Read Height: 9.5 mm
  • Click OK and then Run the experiment.

Result


Header text Header text
100% 43125
50% 16143
25% 7978
12.5% 4124
6.25% 2718
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