User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2012/12/20: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">PcTF Subcloning in E. coli</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">PcTF Subcloning in E. coli</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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'''Table of Components for the IVT reaction''' | '''Table of Components for the IVT reaction''' | ||
{| border="1" | {| border="1" style="border-collapse:collapse;" | ||
! Components !! No DNA Ctrl. (μl) !! GFP Ctrl. (μl) !! Target Protein (μl) with MV2 !! Target Protein (μl) with pT-CHis | ! Components !! No DNA Ctrl. (μl) !! GFP Ctrl. (μl) !! Target Protein (μl) with MV2 !! Target Protein (μl) with pT-CHis | ||
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* The software will deduce the control data from the sample data | * The software will deduce the control data from the sample data | ||
'''RFP result''': | |||
<div class="floatright">[[Image:RFP-In_vitro-PcTF_Expression.png ]]<br>Human in-vitro Protein Expression | |||
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{| border="1" style="border-collapse:collapse;" class="wikitable" | |||
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! Reaction !! RFP (AU) | |||
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| No DNA Negative Control|| 109 | |||
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| GFP Positive Control || 91 | |||
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| Target Protein pC-CHis (Frozen) || 3395 | |||
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| Target Protein pC-CHis (Fresh) || 8819 | |||
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| Target Protein Mv2 || 0 | |||
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Latest revision as of 22:20, 26 September 2017
PcTF Subcloning in E. coli | Main project page Previous entry Next entry | |||||||||||||||||||||||||||||||||||||||||||||||
12/20/20121-Step Human Coupled in vitro protein expression
Table of Components for the IVT reaction
Fluorescent plate reader
RFP result:
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