User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2012/12/20

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12/20/2012

1-Step Human Coupled in vitro protein expression

  • 1-Step Human In Vitro Protein Expression Kits enable the translation and post-transcriptional modification of full-length proteins from mRNA or plasmid templates with yields of up to 100µg/mL per reaction.

Kit Specifications

  • The PcTF gene with two different backbones were used for the protein expression.
    • pCFE1-CHis Expression vector
    • Mv2 vector , Part:BBa_J176122

Table of Components for the IVT reaction

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