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 PcTF Subcloning in E. coli
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12/20/2012
1-Step Human Coupled in vitro protein expression
- 1-Step Human In Vitro Protein Expression Kits enable the translation and post-transcriptional modification of full-length proteins from mRNA or plasmid templates with yields of up to 100µg/mL per reaction.
Kit Specifications
- The PcTF gene with two different backbones were used for the protein expression.
- pCFE1-CHis Expression vector
- Mv2 vector , Part:BBa_J176122
Table of Components for the IVT reaction
| Components |
No DNA Ctrl. (μl) |
GFP Ctrl. (μl) |
Target Protein (μl) with MV2 |
Target Protein (μl) with pT-CHis
|
| Hela Lysate |
43125
|
| Accessory Proteins |
16143
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| Reaction Mix |
7978
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| pCFE-GFP DNA (0.5μg/μL) |
4124
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| Cloned DNA (0.5 μg/μL) |
2718
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| Nuclease free Water |
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PcTF Subcloning in E. coli
