User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2013/01/12: Difference between revisions
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| Ligation || <font color="blue"><u></font> | | Ligation || <font color="blue"><u></font> | ||
|- | |- | ||
| 1. E. coli | | 1. E. coli BL-21 KAH111(E/P)/size, 20 ng + C-His(E/P)/3600, 26ng || <font color="blue">KAH111/C-His 3:1 No Colonies</font> | ||
|- | |- | ||
| 2. E. coli | | 2. E. coli DH5α-T KAH111(E/P)/size, 16 ng + C-His(E/P)/3600, 26ng || <font color="blue">KAH111/C-His 3:1 No Colonies</font> | ||
|- | |- | ||
| | | 3. E. coli BL-21 KAH112(E/P)/size, 22.8 ng + C-His(E/P)/3600, 26ng || <font color="blue">KAH112/C-His 3:1 No Colonies</font> | ||
|- | |- | ||
| | | 4. E. coli DH5α-T KAH111(E/P)/size, 20 ng + C-His(E/P)/3600, 26ng || <font color="blue">KAH112/C-His 3:1 No Colonies</font> | ||
|- | |- | ||
| | | 5. C-His(E/P)/ 26 ng (Control Plate)|| | ||
|} | |} | ||
{| {{table}} cellspacing="3" <!-- Ligation rxn table --> | {| {{table}} cellspacing="3" <!-- Ligation rxn table --> | ||
| || 1 || 2 || 3 | | || 1 || 2 || 3 || | ||
|- | |- | ||
| Insert DNA || 3.12 || 3.12 || --- | | Insert DNA || 3.12 || 3.12 || --- || | ||
|- | |- | ||
| Vector DNA || 2.94 || 2.94 || 2.94 | | Vector DNA || 2.94 || 2.94 || 2.94 || | ||
|- | |- | ||
| 2x lgn buf (Roche) || 7.06 || 7.06 || 5.0 | | 2x lgn buf (Roche) || 7.06 || 7.06 || 5.0 || | ||
|- | |- | ||
| T4 ligase (NEB) || 1.0 || 1.0 || 1.0 | | T4 ligase (NEB) || 1.0 || 1.0 || 1.0 || | ||
|- | |- | ||
| dH<sub>2</sub>O || 0 || 0 || 0.88 | | dH<sub>2</sub>O || 0 || 0 || 0.88 || | ||
|- | |- | ||
| || 14.12 μL || 14.12 μL || 10μl | | || 14.12 μL || 14.12 μL || 10μl || | ||
|} | |} |
Revision as of 14:05, 12 January 2013
PcTF Subcloning in E. coli | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||||||||||||||
01/12/2013Ligation of KAH111, KAH112 and C-His vector in DH5α-T and BL21
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