Revision as of 17:00, 12 January 2013

PcTF Subcloning in E. coli

01/12/2013

Ligation of KAH111, KAH112 and C-His vector in DH5α-T and BL21

The 4 μL of C-His Vector from stock is diluted with 20 μL of nuclease free water, then cut with E/P and purified from the gel.
Ligations

Ligation	</font>
1. E. coli DH5α-T KAH111(E/P)/size, 20 ng + C-His(E/P)/3600, 26ng	KAH111/C-His 3:1 No Colonies
2. E. coli BL-21 KAH111(E/P)/size, 16 ng + C-His(E/P)/3600, 26ng	KAH111/C-His 3:1 No Colonies
1. E. coli DH5α-T KAH112(E/P)/size, 22.8 ng + C-His(E/P)/3600, 26ng	KAH112/C-His 3:1 No Colonies
2. E. coli BL-21 KAH111(E/P)/size, 20 ng + C-His(E/P)/3600, 26ng	KAH112/C-His 3:1 No Colonies
3. C-His(E/P)/ 25 ng

@@ Line 13: / Line 13: @@
 {| {{table}} cellspacing="3" <!-- Ligations table -->
 |- bgcolor=#cfcfcf
-| Ligation || <font color="blue"><u>Plate results (lig : neg crtl)</u> 12/12/12</font>
+| Ligation || <font color="blue"><u></font>
 |-
 | 1. E. coli DH5α-T KAH111(E/P)/size, 20 ng + C-His(E/P)/3600, 26ng || <font color="blue">KAH111/C-His 3:1 No Colonies</font>
 |-
-| 2. E. coli BL-21   KAH111(E/P)/size, 16 ng + C-His(E/P)/3600, 25ng || <font color="blue">KAH111/C-His 3:1 No Colonies</font>
+| 2. E. coli BL-21   KAH111(E/P)/size, 16 ng + C-His(E/P)/3600, 26ng || <font color="blue">KAH111/C-His 3:1 No Colonies</font>
+|-
+| 1. E. coli DH5α-T KAH112(E/P)/size, 22.8 ng + C-His(E/P)/3600, 26ng || <font color="blue">KAH112/C-His 3:1 No Colonies</font>
+|-
+| 2. E. coli BL-21   KAH111(E/P)/size, 20 ng + C-His(E/P)/3600, 26ng || <font color="blue">KAH112/C-His 3:1 No Colonies</font>
 |-
 | 3. C-His(E/P)/ 25 ng ||