User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2013/04/18: Difference between revisions
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[[Image:KAH201-KAH182 Ligation.jpg|thumb| | [[Image:KAH201-KAH182 Ligation.jpg|thumb|400px| (1,2) KAH182, 1675 bp, Cut with Xba1 and Pst1, (3,4) KAH201, 3403 bp, Cut with Spe1 and Pst1]] | ||
{| | {| {{table}} cellspacing="3" width=400px | ||
| Plasmid DNA || 30.00 μl | | Plasmid DNA || 30.00 μl | ||
|- | |- | ||
| Enzyme 1* || 1.0 μl | | Enzyme 1* || 1.0 μl | ||
|- | |- | ||
| Enzyme 2 | | Enzyme 2* || 1.0 μl | ||
|- | |- | ||
| 10x FastDigest buffer + green loading dye || 1.5 μl | | 10x FastDigest buffer + green loading dye || 1.5 μl | ||
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* Enzyme 1 for KAH182 is Xba1 and for KAH201 is Spe1. | * Enzyme 1 for KAH182 is Xba1 and for KAH201 is Spe1. | ||
* Enzyme 2 for KAH182 and KAH201 is Pst1. | |||
Revision as of 22:43, 18 April 2013
PcTF Subcloning in E. coli | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
04/18/2013Building a Reporter Gene to Determine Chromatin Protein Function
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