User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2013/05/06: Difference between revisions
From OpenWetWare
Line 89: | Line 89: | ||
'''Confirm The Assembly''' | '''Confirm The Assembly''' | ||
[[Image: | [[Image:Ligation Confirmation.jpg|thumb|350px| Two seperate bands in each column show the vector backbone and the insert, 4442 bps and the BD001 (KAH201+KAH182) (1901) constructed gene.Backbone, 1901 bps, Cut with SpeI and NotI. First column: Ladder, columns 1-5 are 3:1 ratio colonies, column6 is 4:1 ratio, column 7 is 5:1 ratio and column 8 is 10:1]] | ||
{| {{table}} cellspacing="3" width=400px | {| {{table}} cellspacing="3" width=400px | ||
| Plasmid DNA || 2.0 μl | | Plasmid DNA || 2.0 μl |
Revision as of 13:29, 6 May 2013
PcTF Subcloning in E. coli | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
06/05/2013Building a Reporter Gene to Determine Chromatin Protein Function (Ligation into the mammalian vector)
Confirm The Assembly
|