User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2013/06/06: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">PcTF Subcloning in E. coli</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">PcTF Subcloning in E. coli</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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BD002 (3:1) = 339 ng/μl | BD002 (3:1) = 339 ng/μl | ||
BD002 (4:1) = 317 ng/μl | BD002 (4:1) = 317 ng/μl | ||
KAH187 = 441.5 ng/μl | |||
FlpE = 192 ng/μl | |||
{| {{table}} cellspacing="7" width=700px | {| {{table}} cellspacing="7" width=700px | ||
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| 1-1 || BD002 (3:1) + FlpE || 1.5 + 0.5 μg || 4.42 + 2.60 μL || 12.98μL|| 2.5μL|| 7.5 μL || 570 μL | | 1-1 || BD002 (3:1) + FlpE || 1.5 + 0.5 μg || 4.42 + 2.60 μL || 12.98μL|| 2.5μL|| 7.5 μL || 570 μL | ||
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| 1-2 || BD002 ( | | 1-2 || BD002 (4:1) + FlpE || " || 4.73 + 2.60 μL || 12.67μL|| " || " || " | ||
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| 1-3 | | 1-3 (-) Ctrl || BD002 (3:1) + --- || 2 μg || 4.42 + --- μL || 15.58μL || " || " || " | ||
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| 1-4 | | 1-4 (-)Ctrl || --- + --- || 0 μg || --- + --- μL || 20.0μL|| " || " || " | ||
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|1-5 || | |1-5 || KAH187 || 20 μg || 4.53 || 15.46μL|| " || "||" | ||
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Latest revision as of 22:45, 26 September 2017
PcTF Subcloning in E. coli | Main project page Previous entry Next entry | ||||||||||||||||||||||||||||||||||||||||||||||||
06/06/2013
Minipreps 15 μL --> 37°C/ ~15 min.
1 day before transfection:
Transfections > U2OS Flp-in T-REx lines (puro/zeo/blast) + FRT-marked mammalian transfection vector (hygro)
BD002 (3:1) = 339 ng/μl BD002 (4:1) = 317 ng/μl KAH187 = 441.5 ng/μl FlpE = 192 ng/μl
Transgene expression should be detectable after 18 hours.
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