User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2013/06/17: Difference between revisions
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Transfer [http://openwetware.org/wiki/User:Behzad_Damadzadeh/Notebook/PcTF_Subcloning_in_E-coli/2013/06/10 Transfected Cells] from 6 well plate to 10cm cell culture dishes. | Transfer [http://openwetware.org/wiki/User:Behzad_Damadzadeh/Notebook/PcTF_Subcloning_in_E-coli/2013/06/10 Transfected Cells] from 6 well plate to 10cm cell culture dishes. | ||
Target cell concentrations are: 1*10 | |||
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*Add 2mL Trypsin to each of the wells 1 and 2 for 4 minutes. | *Add 2mL Trypsin to each of the wells 1 and 2 for 4 minutes. | ||
* Add 3.5mL media to neutralize Trypsin. | |||
Revision as of 15:35, 23 July 2013
 PcTF Subcloning in E. coli
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06/17/2013Prepare Transfected Cells for Hygromycine Treatment Transfer Transfected Cells from 6 well plate to 10cm cell culture dishes. Target cell concentrations are: 1*10
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