User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2013/08/05: Difference between revisions
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=Transformation= | =Transformation= | ||
Transformation of Gal4-VP64 parts in '''BL21-DE3'''for transactivation of Cyan flourescent reporter gene. | Transformation of Gal4-VP64 parts in '''BL21-DE3'''for transactivation of Cyan flourescent reporter gene. | ||
'''Parts''' | *'''Parts''' | ||
{| {{table}} cellspacing=''7'' | {| {{table}} cellspacing=''7'' | ||
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| KAH36 || V0120 || [K : mCh : mCh] in pcDNA3.1+/VP64-NLS vector | | KAH36 || V0120 || [K : mCh : mCh] in pcDNA3.1+/VP64-NLS vector | ||
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* 1μL of plasmid and 9μL dH2O. | |||
* 40μL of BL21-DE3 competent cells. | |||
* Culture on AMP agar plates in 37°C incubator overnight. |
Revision as of 11:40, 7 August 2013
PcTF Subcloning in E. coli | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||
08/06/2013TransformationTransformation of Gal4-VP64 parts in BL21-DE3for transactivation of Cyan flourescent reporter gene.
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