User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2013/08/06: Difference between revisions
From OpenWetWare
(Autocreate 2013/08/06 Entry for User:Behzad_Damadzadeh/Notebook/PcTF_Subcloning_in_E-coli) |
(→Date) |
||
| Line 7: | Line 7: | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
= | =08/06/2013= | ||
''' | '''[http://openwetware.org/wiki/User:Behzad_Damadzadeh/Notebook/PcTF_Subcloning_in_E-coli/2013/08/05 Miniprep Transformed Plasmids]''' | ||
# | |||
# Miniprep the transformed plasmids (GAL4-VP64) to test the transfection. | |||
# Pick two colonies of each plate and grow in 2mL of LB broth with 100μg/mL AMP for 10 hrs. | |||
# Miniprep the plasmids with Zyppy Plasmid Miniprep Kit from Zymo | |||
'''Miniprep result''' | |||
{| {{table}} | |||
|- | |||
| Part number || 260 || 280 || 260/280 || ng/ul | |||
|- | |||
| F2620 miniprep 1 || 0.018 || 0.012 || 1.535 || 18.413 | |||
|- | |||
| F2620 miniprep 2 || 0.032 || 0.019 || 1.663 || 32.028 | |||
|- | |||
| J06702 miniprep 1 || 0.009 || 0.006 || 1.367 || 8.633 | |||
|- | |||
| J06702 miniprep 2 || 0.035 || 0.021 || 1.696 || 35.007 | |||
|} | |||
Revision as of 13:57, 8 August 2013
 PcTF Subcloning in E. coli
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||
08/06/2013
Miniprep result
|
||||||||||||||||||||||||||
