User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2013/09/07: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(Autocreate 2013/09/07 Entry for User:Behzad_Damadzadeh/Notebook/PcTF_Subcloning_in_E-coli)
 
Line 7: Line 7:
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->


=Date=
=09/06/2013=


'''List title'''
'''Confirm The Assembly'''
# List items
 
{| {{table}} cellspacing="3" width=800px
|Plasmid Type || SD-BD002<sup>1</sup> || SD-BD002 || SD-BD002|| BD002 (-) Ctrl|| BD002 (+)Ctrl || BD002 (+) Ctrl
|-
| Plasmid DNA || 3.0 μl || 3.0 μl || 3.0 μl || 3.0 μl || 3.0 μl || 3.0 μl
|-
| BsmB1  || 1.0 μl || 0.0 μl || 0.0 μl || 1.0 μl || 0.0 μl || 0.0 μl
|-
| Spe1 || 0.0 μl || 1.0 μl || 0.0 μl || 0.0 μl || 1.0 μl || 0.0 μl
|-
| 10x FastDigest buffer + green loading dye || 1.5 μl || 1.5 μl || 1.5 μl || 1.5 μl || 1.5 μl || 1.5 μl
|-
| dH<sub>2</sub>O || 9.5 μl || 9.5 μl || 10.5 μl || 9.5 μl || 9.5 μl || 10.5 μl
|-
| &nbsp; || 15.0 μl total || 15.0 μl total || 15.0 μl total || 15.0 μl total  || 15.0 μl total || 15.0 μl total
|-
| Incubate at 37°C for 10 minutes.
|}
 
# Make the (1%) agarose glee and add 15μl  of restricted vector in one well and 10 μl  of ladder.
 
[[Image:Image:Ste-Directed-Test.jpg|thumb|350px|]]

Revision as of 12:39, 12 September 2013

PcTF Subcloning in E. coli <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

09/06/2013

Confirm The Assembly

Plasmid Type SD-BD0021 SD-BD002 SD-BD002 BD002 (-) Ctrl BD002 (+)Ctrl BD002 (+) Ctrl
Plasmid DNA 3.0 μl 3.0 μl 3.0 μl 3.0 μl 3.0 μl 3.0 μl
BsmB1 1.0 μl 0.0 μl 0.0 μl 1.0 μl 0.0 μl 0.0 μl
Spe1 0.0 μl 1.0 μl 0.0 μl 0.0 μl 1.0 μl 0.0 μl
10x FastDigest buffer + green loading dye 1.5 μl 1.5 μl 1.5 μl 1.5 μl 1.5 μl 1.5 μl
dH2O 9.5 μl 9.5 μl 10.5 μl 9.5 μl 9.5 μl 10.5 μl
  15.0 μl total 15.0 μl total 15.0 μl total 15.0 μl total 15.0 μl total 15.0 μl total
Incubate at 37°C for 10 minutes.
  1. Make the (1%) agarose glee and add 15μl of restricted vector in one well and 10 μl of ladder.
File:Image:Ste-Directed-Test.jpg
Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Behzad_Damadzadeh/Notebook/PcTF_Subcloning_in_E-coli/2013/09/07&oldid=727879"