User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2013/09/07: Difference between revisions
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=09/06/2013= | =09/06/2013= | ||
'''Confirm The | '''Confirm The Mutagenesis''' | ||
{| {{table}} cellspacing="3" width=800px | {| {{table}} cellspacing="3" width=800px | ||
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| Plasmid DNA || 3.0 μl || 3.0 μl || 3.0 μl || 3.0 μl || 3.0 μl || 3.0 μl | | Plasmid DNA || 3.0 μl || 3.0 μl || 3.0 μl || 3.0 μl || 3.0 μl || 3.0 μl | ||
|- | |- | ||
| | | BsmBI || 1.0 μl || 0.0 μl || 0.0 μl || 1.0 μl || 0.0 μl || 0.0 μl | ||
|- | |- | ||
| Spe1 || 0.0 μl || 1.0 μl || 0.0 μl || 0.0 μl || 1.0 μl || 0.0 μl | | Spe1 || 0.0 μl || 1.0 μl || 0.0 μl || 0.0 μl || 1.0 μl || 0.0 μl | ||
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# Make the (1%) agarose glee and add 15μl of restricted vector in one well and 10 μl of ladder. | # Make the (1%) agarose glee and add 15μl of restricted vector in one well and 10 μl of ladder. | ||
* The gel picture shows that the BSMBI cut site did not remove. | |||
Revision as of 08:59, 13 October 2013
 PcTF Subcloning in E. coli
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09/06/2013Confirm The Mutagenesis
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