User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2013/10/10: Difference between revisions
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** [95°C/ 30 sec; 55°C/ 1 min; 72°C/ '''6''' min (1 min/kb plasmid length)]x30 cycle | ** [95°C/ 30 sec; 55°C/ 1 min; 72°C/ '''6''' min (1 min/kb plasmid length)]x30 cycle | ||
** 4°C, ∞ | ** 4°C, ∞ | ||
* Run 5 μL of each PCR reaction on 10% agarose gel. | |||
[[Image:Image:BD002-SD-SAPI.png]] |
Revision as of 09:48, 13 October 2013
PcTF Subcloning in E. coli | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||
10/10/12Site Directed Mutagenesis-Removing the BSMBI cut site from BD002 reporter gene
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