User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2013/10/17: Difference between revisions
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== Golden Gate Assembly of BD002 to Replace HsvtkTATA with CMV and HPK Promoters== | == Golden Gate Assembly of BD002 to Replace HsvtkTATA with CMV and HPK Promoters== | ||
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Existing construct is: 5XGAL4-Spacer-'''HsvTK'''-Kozak-AMCyan-AmCyan-NLS-Stop in V0200 [http://parts.igem.org/Part:BBa_J176121?title=Part:BBa_J176121 mammalian vector] | Existing construct is: 5XGAL4-Spacer-'''HsvTK'''-Kozak-AMCyan-AmCyan-NLS-Stop in V0200 [http://parts.igem.org/Part:BBa_J176121?title=Part:BBa_J176121 mammalian vector] | ||
Amplification of CMV and HPK from [http://parts.igem.org/Part:BBa_J176094 KAH187] and [http://parts.igem.org/Part:BBa_J176092 KAH184] respectively. |
Revision as of 14:57, 24 October 2013
PcTF Subcloning in E. coli | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Golden Gate Assembly of BD002 to Replace HsvtkTATA with CMV and HPK PromotersDesired Constructs: BD003: 5XGAL4-Spacer-CMV-Kozak-AMCyan-AmCyan-NLS-Stop BD004: 5XGAL4-Spacer-HPK-Kozak-AMCyan-AmCyan-NLS-Stop Existing construct is: 5XGAL4-Spacer-HsvTK-Kozak-AMCyan-AmCyan-NLS-Stop in V0200 mammalian vector Amplification of CMV and HPK from KAH187 and KAH184 respectively. |