User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2013/10/29: Difference between revisions
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| align="center" style="background:#f0f0f0;"|'''Thermal Cycling Reaction Conditions''' | | align="center" style="background:#f0f0f0;"|'''Thermal Cycling Reaction Conditions''' | ||
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| 20 fmol of each DNA part (µL)||1+1||1+1||1|| 1 || 1 || rowspan=' | | 20 fmol of each DNA part (µL)||1+1||1+1||1|| 1 || 1 || rowspan='7' | | ||
* [45°C, 2 min.; 16°C 5 min.] x25 | * [45°C, 2 min.; 16°C 5 min.] x25 | ||
* 60°C, 10 min. | * 60°C, 10 min. |
Revision as of 14:38, 30 October 2013
PcTF Subcloning in E. coli | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||||||
Golden Gate Assembly of BD002 to Replace HsvtkTATA with CMV and HPK Promoters (Third Try)
Backbone vector [math]\displaystyle{ X= (4600 / 209 ) * 0.013 * 20 = 5.72 }[/math] HPK [math]\displaystyle{ X= (516 / 70 ) * 0.013 * 20 = 1.91 }[/math], [math]\displaystyle{ 1.91 + 18.1 = 20 }[/math] CMV [math]\displaystyle{ X= (588 / 42 ) * 0.013 * 20 = 3.64 }[/math], [math]\displaystyle{ 3.64 + 16.36 = 20 }[/math]
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