User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2013/11/13: Difference between revisions
From OpenWetWare
(→Date) |
(→Date) |
||
Line 30: | Line 30: | ||
| 11/11/2013||Plasmid: BD002||[95C (30 sec.), 49C (45 Sec.), 72C (6 min.)]||[[Image:20131110 213024 (2).jpg |250px]]||Column 1,2: 9 ng Plasmid, Column 3,4: 0.09 ng Plasmid, Column 5,6: 0.5 ml primer from 10mM solution | | 11/11/2013||Plasmid: BD002||[95C (30 sec.), 49C (45 Sec.), 72C (6 min.)]||[[Image:20131110 213024 (2).jpg |250px]]||Column 1,2: 9 ng Plasmid, Column 3,4: 0.09 ng Plasmid, Column 5,6: 0.5 ml primer from 10mM solution | ||
|- | |- | ||
| 11/11/2013||0.09ng Plasmid: BD002||[95C (30 sec.), 49C (45 Sec.), 75C (6 min.)]||||Increase the extention temperature to 75C and annealing temperature to 49C | | 11/11/2013||0.09ng Plasmid: BD002||[95C (30 sec.), 49C (45 Sec.), 75C (6 min.)]||[[Image:20131110 213024 (3).jpg |250px]]||Increase the extention temperature to 75C and annealing temperature to 49C | ||
|- | |- | ||
| 11/11/2013||0.09ng Plasmid: BD002||[95C (30 sec.), 55C (45 Sec.), 72C (6 min.)]||||Increase the annealing temperature to 55C | | 11/11/2013||0.09ng Plasmid: BD002||[95C (30 sec.), 55C (45 Sec.), 72C (6 min.)]||||Increase the annealing temperature to 55C | ||
|- | |- | ||
|} | |} |
Revision as of 13:38, 13 November 2013
PcTF Subcloning in E. coli | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
DateTroubleshooting BD002 PCR Linearization for GG Assembly
|