User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2013/11/13: Difference between revisions
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| 11/9/2013||9 ng Plasmid: KAH187 (CMV)||[95C (30 sec.), 45C (45 Sec.), 72C (6 min.)]||[[Image:20131109 123553 (3).jpg |250px]]|| | | 11/9/2013||9 ng Plasmid: KAH187 (CMV)||[95C (30 sec.), 45C (45 Sec.), 72C (6 min.)]||[[Image:20131109 123553 (3).jpg |250px]]|| | ||
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| 11/10/2013||0.09ng Plasmid: BD002||[95C (30 sec.), 45C (45 Sec.), 72C (6 min.)]||||Gel Extract the band close to 5K bps No result | | 11/10/2013||0.09ng Plasmid: BD002||[95C (30 sec.), 45C (45 Sec.), 72C (6 min.)]||[[Image:20131110 122904 (2).jpg |250px]]||Gel Extract the band close to 5K bps No result | ||
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| 11/10/2013||0.09ng Plasmid: BD002||[95C (30 sec.), 45C (45 Sec.), 72C (6 min.)]||||Amplified with V0200 Sequencing Primers to make sure Kozak-HSV-CFP-NLS-Stop-PolyA (2K bps are there). | | 11/10/2013||0.09ng Plasmid: BD002||[95C (30 sec.), 45C (45 Sec.), 72C (6 min.)]||||Amplified with V0200 Sequencing Primers to make sure Kozak-HSV-CFP-NLS-Stop-PolyA (2K bps are there). |
Revision as of 13:26, 13 November 2013
PcTF Subcloning in E. coli | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
DateTroubleshooting BD002 PCR Linearization for GG Assembly
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