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Objectives
- Set up 1ml for AgPea Protein
- Set up 20ml AgNP and AuNP reactions for Casein, Soy, Whey, Rice
Materials
- Used same nutritional proteins on 02/02/16
- Protein and gold stocks prepared fresh every day
- Ocean Optics UV Vis was used to record UVVIS Spectra of Casein AgNPs. The other machine broke and is unusable.
- Integration time= 10ms and Average =100.
- A water blank in the same quartz cuvette was subtracted from the spectra before running any samples.
Procedure
- Further reduce stock protein concentrations (and thus concentrations in reaction mix) to increase metal/protein ratio to spur NP formation as we saw protein aggregate and nanofiber formation in previous reactions
- increase concentration from 02/16/16 trial
- comparing citrate to no citrate samples, and vary citrate concentration
- Prepare 20ml reaction to synthesize large quantities of reactions known to work
- Pea Protein and Brown Rice Protein AgNP
- Note that spectra of reaction mixes before reacting need to be taken
- Also note that this is just one size of nanoparticle that can be made with these reactions, its possible to change variables and change size and functionality of nanoparticle, these were just the first reactions to work.
- Reaction mix data can be found at the link SRB Lab Notebook Data
Results
- Color change notes and UV-VIs information can be found at the link SRB Lab Notebook Data
- It was noted that diluting Pea Protein in base allowed for the silver nanoparticle reaction. From now on, will dilute pea protein in base. This is similar to what was experienced with brown rice protein and casein - both of which were originally dissolved in base and synthesized silver nanoparticles.
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Project name
