User:Benjamin Friedel/Notebook/CHEM 471/2016/03/01: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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==Procedure== | ==Procedure== | ||
Materials for Agar Disc Diffusion | |||
#Mueller Hinton Agar | #Mueller Hinton Agar | ||
-Dissolved Agar packet in 1L of water over hot plate | |||
-Autoclaved on wet cycle | |||
## | ## Whatman Membrane Filter Paper | ||
-autoclaved in dry cycle | |||
### Protein-Nanoparticle samples and Ampicillin Control Dilutions | |||
AMP Table | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Concentration (mg/L)''' | |||
| align="center" style="background:#f0f0f0;"|'''Concentration (ug/uL)''' | |||
| align="center" style="background:#f0f0f0;"|'''Volume (ul)''' | |||
| align="center" style="background:#f0f0f0;"|'''Mass (ug)''' | |||
|- | |||
| 4||0.004||25||0.1 | |||
|- | |||
| 8||0.008||25||0.2 | |||
|- | |||
| 16||0.016||25||0.4 | |||
|- | |||
| 32||0.032||25||0.8 | |||
|- | |||
| 64||0.064||25||1.6 | |||
|} | |||
Example Nanoparticle Sample Dilutions | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Concentration (x)''' | |||
|- | |||
| 1 | |||
|- | |||
| 0.5 | |||
|- | |||
| 0.25 | |||
|- | |||
| 0.125 | |||
|- | |||
| 0.0625 | |||
|} | |||
# Reaction mix data can be found at the link [https://docs.google.com/spreadsheets/d/1XIncoYXZyIeGlpAFkbnr44U3PQ61jNbax-hkexNerUM/edit#gid=303734626 SRB Lab Notebook Data] | # Reaction mix data can be found at the link [https://docs.google.com/spreadsheets/d/1XIncoYXZyIeGlpAFkbnr44U3PQ61jNbax-hkexNerUM/edit#gid=303734626 SRB Lab Notebook Data] | ||
Due to time constraints, the actual diffusion assay was not performed this day | |||
Latest revision as of 01:43, 27 September 2017
Project name | Main project page Previous entry Next entry | ||||||||||||||||||||||||||||||
Objectives
Materials
ProcedureMaterials for Agar Disc Diffusion
-Dissolved Agar packet in 1L of water over hot plate -Autoclaved on wet cycle
-autoclaved in dry cycle
AMP Table
Due to time constraints, the actual diffusion assay was not performed this day
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