User:Benjamin Friedel/Notebook/CHEM 471/2016/03/23: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
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#Mueller Hinton Agar pre-prepared and stored in fridge | #Mueller Hinton Agar pre-prepared and stored in fridge | ||
#Whatman Membrane Filter Paper (6mm discs punched out from larger discs) | |||
-autoclaved in dry cycle | -autoclaved in dry cycle | ||
# Dilutions of Protein Nanoparticle Samples and Ampicillin Control | #Dilutions of Protein Nanoparticle Samples and Ampicillin Control | ||
#Protein-Nanoparticle samples and Ampicillin Control Dilutions | |||
AMP Table | AMP Table |
Latest revision as of 01:43, 27 September 2017
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Objectives
ProcedureMaterials for Agar Disc Diffusion
-autoclaved in dry cycle
AMP Table
Disc Diffusion Procedure
Note that this technique was conducted on the benchtop. Between each loading of a disc on a plate, the tweezers used were washed with EtOH to minimize contamination. However, the plates were open to the air for a period of time during plating and loading of discs. Because we are testing for bacterial inhibition by the nanoparticles and control, if there is contamination, but inhibition we may never know that there was contamination in the first place. Discussed with Dr. Hartings and protocol was approved.
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