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HOG Experiments : wild - type controls
Objective
- Make sure I can do HOG experiments again
- Confirm Wild-type values and also expand the frequencies tested
- Confirm the protocol: specifically that waiting 30 minutes between each set of oscillations is enough to ensure that the cells return to normal
- See if the cells are growing
Experiment
- Cells OD @ 10:30 = .389
- Started flow at 11 am
- Started experiment at 11:30 am
Settings
- 240 second half-period
- 48 second imaging = 6 images per cycle
- 40 minute total time = 5 complete periods
- ND=2
- mCherry = 100 mS exposure
- GFP = 200 mS exposure
- DIC
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